Soluble invasion plasmid antigen C (IpaC) from Shigella flexneri elicits epithelial cell responses related to pathogen invasion

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Soluble invasion plasmid antigen C (IpaC) from Shigella flexneri elicits epithelial cell responses related to pathogen invasion.

Shigella flexneri invades colonic epithelial cells by pathogen-induced phagocytosis. The three proposed effectors of S. flexneri internalization are invasion plasmid antigens B (IpaB), IpaC, and IpaD, which are encoded on the pathogen's 230-kb virulence plasmid and translocated to the extracellular milieu via the Mxi-Spa translocon. To date, there are no definitive functional data for any purif...

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Structure-function analysis of invasion plasmid antigen C (IpaC) from Shigella flexneri.

Shigella flexneri causes a self-limiting gastroenteritis in humans, characterized by severe localized inflammation and ulceration of the colonic mucosa. Shigellosis most often targets young children in underdeveloped countries. Invasion plasmid antigen C (IpaC) has been identified as the primary effector protein for Shigella invasion of epithelial cells. Although an initial model of IpaC functi...

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Characterization of invasion plasmid antigen genes (ipaBCD) from Shigella flexneri.

The large invasion plasmid of Shigella flexneri M9OT-W was used to generate recombinant plasmids carrying the ipaA, -B, -C, and -D genes, whose products are associated with the entry of the bacteria into colonic epithelial cells. Complete DNA sequences of ipaB, -C, and -D were determined. The proteins predicted (62, 42, and 37 kDa, respectively) from the nucleotide sequences lack a signal-pepti...

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Probiotic Bifidobacterium Lactis Bacteria Inhibit the Invasion Phenotype of Shigella Dysenteriae Induced By Invasion Plasmid Antigen C

Background and Aims: Shigellosis is an acute gastroenteritis and Invasion plasmid antigen C (IpaC) is the first effector protein for Shigella invasion of intestinal cells. Among lactic acid bacteria, Bifidobacterium lactis (B. lactis) has received increasing attention for protection of a potential host against gastrointestinal infections. The aim of this study was to investigate the inhibitory ...

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Identification of epitope and surface-exposed domains of Shigella flexneri invasion plasmid antigen D (IpaD).

Transport and surface expression of the invasion plasmid antigens (Ipa proteins) is an essential trait in the pathogenicity of Shigella spp. In addition to the type III protein secretion system encoded by the mxi/spa loci on the large virulence plasmid, transport of IpaB and IpaC into the surrounding medium is modulated by IpaD. To characterize the structural topography of IpaD, the Geysen epit...

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ژورنال

عنوان ژورنال: Infection and Immunity

سال: 1996

ISSN: 0019-9567,1098-5522

DOI: 10.1128/iai.64.10.4182-4187.1996